Figure 1

Effect of Ax-hp16 or Ax-hp21 infection on cell survival or cell viability in p16- or p21-null human glioma cell lines with or without irradiation. At 1 day after irradiation, U251MG cells (p16-null, p21-null, p53-mut) were infected with adenovirus vector at 5 MOI, and D54MG cells (p16-null, p53-wild) were infected at 10 MOI. (A) U251MG, Ax-mock or Ax-hp16 infection; (B) D54MG, Ax-mock or Ax-hp16 infection; and (C) U251MG, Ax-mock or Ax-hp21 infection show cell survival determined by clonogenic assays. (D) U251MG, Ax-mock or Ax-hp16 infection and (E) D54MG, Ax-mock or Ax-hp16 infection show cell viability determined using Trypan blue exclusion. The results of cell viability assays are shown as the mean number of cells at each point, with standard deviation of three wells. Statistical significance: ^*P<0.05, compared with cells other than irradiated Ax-hp16-infected cells.