Figure 4
Effect of p16 or p21 transduction with or without irradiation on nuclear morphologic changes. U251MG or D54MG cells were treated as described in the TUNEL assay, and nuclear morphologic changes in these treated cells were evaluated using Giemsa staining 7 days after irradiation. The frequency of abnormal nuclei (including nuclear budding, micronucleation, binucleation and multinucleation) in U251MG (A) or D54MG cells (B) is expressed in comparison with 1000 culture cells with a single nucleus (ie, uninucleated cells). The figures show (C) multinucleation, (D) micronucleation (one arrow) and nuclear budding (double arrows) in irradiated Ax-hp16-infected U251MG cells, and (E) micronucleation (one arrow), nuclear budding (two arrows) and multinucleation (triple arrows) in irradiated Ax-hp16-infected D54MG cells.
