Figure 6
Western blot of active (phosphorylated) ERK1/2 and total ERK1/2 (p44 and p42) (B) in soluble extracts of C2C12 myotubes treated with 0 (lanes 1 and 7); 1.0 (lanes 2 and 8); 2.1 (lanes 3 and 9); 4.2 (lanes 4 and 10); 10 (lanes 5 and 11) or 20 nM PIF (lanes 6 and 12), without pretreatment (lanes (1–6) or after pretreatment for 2 h in the presence of 10 μ M PD98059 (lanes 7–12).
