Figure 3
From: Multiple and synergistic deregulations of apoptosis-controlling genes in pancreatic carcinoma cells

Expression of DISC interacting proteins FADD and FLIP in pancreatic tumour cells. Equal amounts of whole cell lysates (30 μg) from each cell line were separated in 4–12% gradient gels and blotted onto PVDF membrane. Subsequently, the expression of FADD and FLIP was detected using specific antibodies. Equal loading of slots was verified by determination of β-actin expression. Data are representative of at least three independent experiments.