Figure 5
From: Multiple and synergistic deregulations of apoptosis-controlling genes in pancreatic carcinoma cells

Immunoblot detection of proteins of the IAP-family in pancreatic tumour cells. Protein extracts (30 μg) of pancreatic tumour cells were separated by 12% SDS–PAGE, blotted onto PVDF membranes and the expression of c-IAP2, XIAP and Survivin was analysed using specific antibodies. In parallel, β-actin expression was determined in all lanes. Representative results of three independent experiments are shown.