Figure 6
From: Multiple and synergistic deregulations of apoptosis-controlling genes in pancreatic carcinoma cells
Anti-CD95 and TRAIL sensitivity of pancreatic adenocarcinoma cells overexpressing Bcl-xL (A), XIAP (B), truncated FADD (tFADD) (C) and Bid (D). Upper parts of each figure show the expression of Bcl-xL, XIAP, tFADD or Bid determined by Western blot in cell pools retrovirally transduced (Bcl-xL, tFADD and Bid) or transfected (XIAP) with vectors bearing the corresponding genes (see material and methods). As control, wild-type-cells and cells transfected with an empty vector were analysed in parallel. Lower parts of each figure show the quantification of cellular DNA-fragmentation by the JAM-Assay in cell pools treated for 24 h with anti-CD95 (100 ng ml−1) or TRAIL (100 ng ml−1). Data represent means±SD (n=6) from one representative experiment out of at least three performed.
