Table 1 Genes differentially expressed in parental LoVo cells and c-Myc-overexpressing L2 and L3 isogenic cells

From: c-Myc overexpression sensitises colon cancer cells to camptothecin-induced apoptosis

   

Microarray a

RT–PCR b

Correlation c

Accession

Gene name

Function

L2/L

L3/L

L2/L

L3/L

r

P

AA088861

Cadherin 17, LI cadherin (liver–intestine)

Cell adhesion

0.23

0.18

0.006

0.023

−0.16

 

AA130579

Lectin, galactoside-binding, soluble, 4 (galectin 4)

Cell adhesion

7.14

10.89

  

0.33

 

N30868

Integrin, alpha 4

Cell adhesion

4.75

6.87

  

0.48

0.016

R60995

Coagulation factor C homologue, cochlin

Cell communication

0.12

0.17

  

−0.54

0.002

AA453783

Mal, T-cell differentiation protein 2

Development–differentiation

6.26

4.5

  

0.35

 

H17696

Myelin basic protein

Development–differentiation

12.03

10.41

0.260

0.350

−0.09

 

AA290737

Glutathione S-transferase M1

Drug metabolism/resistance

0.17

0.25

0.002

0.012

−0.43

0.019

H29521

ATP-binding cassette 3

Drug metabolism/resistance

0.14

0.19

0.003

0.001

−0.09

 

H88329

Calbindin 1, (28 kDa)

Drug metabolism/resistance

0.11

0.1

0.004

0.001

0.00

 

AA664101

Aldehyde dehydrogenase 1 family, member A1

Drug metabolism/resistance

44.09

28.97

832

1137

0.49

0.006

AA031513

Matrix metalloproteinase 7 (matrilysin, uterine)

Extracellular matrix

20.68

17.23

  

−0.06

 

AA056013

Microfibril-associated glycoprotein-2

Extracellular matrix

0.19

0.2

  

0.11

 

AA479199

Nidogen 2

Extracellular matrix

0.21

0.22

0.004

0.002

−0.14

 

AA458965

Natural killer cell transcript 4

Immune/inflammatory

0.08

0.16

  

−0.07

 

AA682631

Calcineurin A alpha

Kinase/phosphatase

0.23

0.24

  

−0.28

 

AA431773

Fatty acid desaturase 1

Lipid metabolism

0.18

0.21

0.005

0.007

−0.59

0.001

AA432066

Sarcoglycan, epsilon

Metabolism

0.23

0.2

  

−0.12

 

AA434024

Lanosterol synthase

Metabolism

0.23

0.24

0.101

0.210

−0.14

 

AA401137

Lipocalin 2 (oncogene 24p3)

Oncogene

10.98

8.89

  

0.12

 

AA045436

Basic leucine zipper transcription factor MafG

Oncogene

5.07

4.33

1.370

4.820

−0.15

 

N63943

Lysozyme (renal amyloidosis)

Antimicrobial

10.01

8.18

2161.8

1817.5

0.55

0.002

AA134814

TRAF family member-associated NFKB activator

Signal transduction

4.83

4.44

  

0.55

0.013

AA598567

Myelin gene expression factor 2

Transcriptional coactivator

5.02

4.85

  

−0.05

 

AA055486

Tripartite motif-containing 29

Transcription factor

5.35

4.84

  

−0.10

 

AI017703

Eukaryotic translation initiation factor 3, subunit 3

Translation/AA biochemistry

5.11

4.25

  

0.45

0.013

R38343

Protein tyrosine phosphatase, receptor type, G

Transmembrane receptor

0.23

0.18

  

−0.16

 

T89391

Caveolin 2

Tumour suppressor

0.19

0.22

0.025

0.067

−0.01

 

AA112057

KIAA0143 protein

Unknown

6.73

5.57

  

0.40

0.031

AA282134

OK/SW-cl.68 mRNA, complete cds

Unknown

16.06

10.51

774.2

568.4

0.12

 

AA702350

Autism-related protein 1

Unknown

0.06

0.08

  

−0.04

 

AA702949

KIAA0443 gene product

Unknown

0.11

0.16

  

−0.06

 

R40481

cDNA FLJ34699 fis, clone MESAN2002186

Unknown

6.57

4.55

  

0.39

0.037

T62854

Hypothetical protein FLJ22662

Unknown

0.14

0.19

  

0.06

 

W16832

Muscleblind-like protein MBLL39

Unknown

4.48

4.93

39.1

87.0

0.19

 
  1. Genes with significant correlations between their expression levels and apoptotic response to camptothecin in a panel of 30 colorectal cancer cell lines are shown in bold font.
  2. aThe relative mRNA levels are shown for the 34 genes with a difference in expression greater than four-fold in LoVo vs L2 and L3 in the microarray experiments (mean of two experiments).
  3. The relative expression levels are measured using quantitative real-time RT–PCR for 14 of these 34 genes.
  4. Pearson's correlation for gene expression and response to camptothecin in 30 colorectal cancer cell lines.
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