Figure 4
Intracellular calcium responses of (1) HBL (2) A375-SM and (3) C8161 melanoma cells to α-MSH+PIA (10 μ M). Cells were loaded with Fura-2 AM and the intracellular calcium release was detected by optical fluorimetry. +(−)-N6-(2-phenylisopropyl)-adenosine was always added at the same time as α-MSH indicated by position of individual vertical arrows. (a) α-MSH (10−11 M); (b) α-MSH (10−9 M) and (c) α-MSH (10−7 M).
