Table 1 Studies using the ACA to measure DNA damage and repair in tumour cells following exposure to X-irradiation
From: Potential use of the alkaline comet assay as a predictor of bladder tumour response to radiation
Radiation Dose range/time of repair study | ||||
|---|---|---|---|---|
Reference | Sample | ID a | Repair | Main conclusions |
Olive et al (1990) | Mouse tumour cells (*SCC VII) and tumour-derived macrophages | 0–15 Gy | 15 Gy 0–30 min | ID: increased linearly with dose, similar in normal and tumour cells Repair: no difference |
Müller et al (1994) | Human SCCs (PECA 4451, PECA 4197), human melanoma (MeWo) | 0–2 Gy | 5 Gy 0–120 min | ID: no correlation Repair: Some correlation |
McKelvey-Martin et al (1998) | Three human bladder tumour cell lines (RT112, UM-UC-3, HT1376) | 0–10 Gy | ND | ID: inverse correlation SF2 and tail moment |
Bergqvist et al (1998) | Four human lung tumour cell lines (U-1285, U-1906E, U-1752, U-1810) | 0–5 Gy | 0–5 Gy 60 min | ID: No correlation with SF2 RD: some correlation at 60 min |
Bachova et al (2002) | Three human ovarian tumour cell lines (CH-1, A-2780, SKOV-3) | 0, 2, 8 Gy | 2 and 8 Gy 0–90 min | ID: correlation with apoptosis Repair rate: no correlation RD: some correlation at 30 min |
