Table 1 Summary of caspase processing induced by cytochrome c in extracts from RCC cell lines

From: Functional evaluation of the apoptosome in renal cell carcinoma

 

KTCTL-26A

KTCTL-30

CAKI-1

KTCTL-1M

KTCTL-84A

Cytochrome c (μg ml−1)

1

50

1

50

1

50

1

50

1

50

Caspase-3

0/1/1

2/1/2

1/1/2

2/2/2

1/1/1

2/1/2

1/1/1

2/2/2

1/1/1

1/2/1

Caspase-9

1/2

3/3

3/3

3/3

2/3

3/3

3

3

2/2

3/3

Caspase-7

1/3/3

3/3/3

3/3/3

3/3/3

3/3/3

3/3/3

3/3/3

3/3/3

2/3/1

3/3/3

  1. Extracts from five RCC cell lines were incubated in the absence of cytochrome c or in the presence of 1 or 50 μg ml−1 cytochrome c for 1 h at 37°C. Reactions were then tested by immunoblotting for processing of caspase-3, -7 and -9. Semiquantitative values were allocated as follows: (0) no processing detectable; (1) processed caspase visible but no visible loss of procaspase; (2), decrease in the signal of the procaspase band visible; and (3), procaspase completely disappeared. Experiments were performed three times for caspase-3 and -7, one or two times for caspase-9. Each value represents one separate experiment.
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