Table 2 Induction of DEVD-cleaving activity and caspase processing induced by cytochrome c in normal kidney cells

		Caspase-3		Caspase-7		Caspase-9		Apaf-1
Patient	DEVD-cleaving activity ^* (% standard)	Expr.	Proc.	Expr.	Proc.	Expr.	Proc.	Expr.
#65	0.8	++	0	+++	0	+	0	NT
#66	38.8	++	2	+	3	+	1	NT
#70	65.0	++	1	+	2	++	2	++
#71	197.9	++	2	−	0	++	2	++
#78	32.0	+	2	+	2	+	2	NT

Extracts from five samples of isolated normal kidney cells were incubated with cytochrome c (50 μg ml⁻¹) as above for 1 h at 37°C. DEVD-cleaving activity was measured and is shown as percent of HeLa cell extract control. Processing of caspases and the presence of Apaf-1 in extracts was assessed by immunoblotting. Semiquantitative assessment of processing was as follows: (0) no detectable expression; (1) levels less than; (2) levels equal to; and (3) levels higher than in HeLa cells (see also Materials and Methods). NT=not tested. ^*DEVD-cleaving activity, effect of caspase activity (see Methods).

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