Figure 1 | British Journal of Cancer

Figure 1

From: Analysis of the expression and localisation of a LAP protein, human scribble, in the normal and neoplastic epithelium of uterine cervix

Figure 1

Construction of the anti-hScrib antibody using its C-terminus. (A) Structure of hScrib consists of 16 canonical LRRs, followed by LAPSD (LAP-specific domain) and four PDZ domains. We generated an anti-hScrib antibody (anti-hScrib C-terminus) using C-terminus (AA 1208–1630) as antigens. (B) Anti-hScrib C-terminus antibody detected the endogenous hScrib protein with 220 kDa in molecular weight in 293 T cells (lane 1) and in vitro-translated hScrib (lane 2), but not in vitro-translated hDlg by Western blotting (lane 3).

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