Figure 5

Analyses of apoptosis in KPL-3C and MDA-MB-231 cells treated with E2, fulvestrant and/or gefitinib. Semi-confluent cells were cultured for 4 days in the oestrogen-deprived medium with vehicle (control), 1 nM E2 plus 100 nM fulvestrant and 10 μ M gefitinib or 10 μ M gefitinib alone. The percentages of apoptotic cells were measured with a flow cytometry using an in situ cell death detection kit. Note the increase in apoptotic cells by the addition of fulvestrant and gefitinib to E2 in KPL-3C cells and in MDA-MB-231 cells treated with gefitinib alone.