Figure 3
The EGF-induced migration of MDA-HER2 cells is permanently blocked by PS2-TAT. Cell migration was analysed by using the three- dimensional collagen matrix assay combined with computer-assisted cell tracking. (A) The mean locomotory activity of EGF (100 ng ml−1)-treated MDA-HER2 cells was significantly (*=P<0.0001) reduced by about 75% by PS2-TAT (2 μ M) treatment. Similar results were achieved by U73122 (2 μ M) that was used as a control. In contrast to this, PS1-TAT (2 μ M) showed a rather marginal inhibition of the EGF (100 ng ml−1)-induced migration of MDA-HER2 cells. The graph represents the mean locomotory activity of treated and untreated MDA-HER2 cells of four independent experiments (120 cells). (B) The Box-Plot diagram represents the time locomotion of single MDA-HER2 cells. PS2-TAT (2 μ M) but not PS1-TAT (2 μ M) treatment resulted in a markedly reduced time locomotion of EGF (100 ng ml−1)-treated MDA-HER2 cells. U73122 (2 μ M) treatment yielded comparable results. Results were obtained from four independent experiments (120 cells).
