Figure 1 | British Journal of Cancer

Figure 1

From: Tissue inhibitor of metalloproteinase-1 stimulates proliferation of human cancer cells by inhibiting a metalloproteinase

Figure 1

Inhibition of MMP-1 by purified TIMP-1 with inset figure of silver-stained SDS–PAGE and Western blot of purification steps for TIMP-1. Concentration-dependent inhibition of human fibroblast collagenase activity by purified human TIMP-1. The TIMP-1 concentration of 750 ng corresponds to a 1 : 1 molar ratio of enzyme to inhibitor. The inset consists of two parts; the top part is a silver stain of the fractions from each step in the purification of hTIMP-1. Lane 1 contains medium conditioned by transfected insect cells; lane 2 contains pooled fractions from a nickel column; lane 3 contains pooled fractions from an SP-cation exchange column; and lane 4 shows the electroeluted human TIMP-1. The bottom part is a Western blot of the same fractions showing a single band corresponding to hTIMP-1.

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