Figure 1
From: Expression of MTA1 promotes motility and invasiveness of PANC-1 pancreatic carcinoma cells
Northern blot analysis of MTA1 mRNA expression in pancreatic carcinoma cell lines. Total RNA was prepared from PANC-1, BxPC-3, MiaPaCa-2, PaTu 8988 s, and PaTu 8988t pancreatic carcinoma cells, fractionated (30 μg lane−1) by denaturating formaldehyde–agarose (1% (w v−1)) gel electrophoresis and transferred onto a nylon membrane. Hybridisation was performed with a radiolabelled MTA1 cDNA probe (upper panel). To assess equal loading of the analysed RNA, the blot was rehybridised with a radiolabelled β-actin cDNA fragment (lower panel). The positions of the 28S and 18S ribosomal RNA bands are indicated.
