Figure 4

(A) HPLC analysis of liver extracts of mice that received resveratrol (240 mg kg⁻¹) p.o. (i) and of a mixture (ii) of (i) and biosynthesised resveratrol glucuronide. Liver tissue was obtained 60 min postadministration. Peak allocation is (1) resveratrol glucuronide, (2) resveratrol sulphate and (3) resveratrol. The chromatogram is representative of three. For details of dosing, extraction and HPLC analyses, see Materials and Methods. (B) HPLC analysis of liver extracts of mice that received DMU 212 (240 mg kg⁻¹) p.o. (i) a mixture (ii) of (i) with authentic standards DMU 212, 4,4′-di-desmethyl-DMU 212 (DMU 295), 4′-desmethyl-DMU 212 (DMU 281), 3′-hydroxy-DMU 212 (DMU 214), 4-desmethyl-DMU 212 (DMU 291) and 3-desmethyl-DMU 212 (DMU 807). Liver tissue was obtained 60 min postadministration. Peak allocation is (4) 4,4′-di -desmethyl-DMU 212 (DMU 295) (5) 4′-desmethyl-DMU 212 (DMU 281), (6) 3′-hydroxy-DMU 212 (DMU 214), (7) 4-desmethyl-DMU 212 (DMU 291), (8) 3-desmethyl-DMU 212 (DMU 807) (9) DMU 212 and (10) internal standard. The chromatogram is representative of three. For details of dosing, extraction and HPLC analyses, see Materials and Methods. (C) HPLC analysis of extracts of an incubate of mouse liver microsomes with DMU 212 (1 mM) (i) and of a mixture (ii) of (i) with authentic DMU 212, 4,4′-di-desmethyl-DMU 212 (DMU 295), 4′-desmethyl-DMU 212 (DMU 281), 3′-hydroxy-DMU 212 (DMU 214), 4-desmethyl-DMU 212 (DMU 291) and 3-desmethyl-DMU 212 (DMU 807). Incubations were terminated after 20 min. Peak allocation is (4) 4,4′-di-desmethyl-DMU 212 (DMU 295) (5) 4′-desmethyl-DMU 212 (DMU 281), (6) 3′-hydroxy-DMU 212 (DMU 214), (7) 4-desmethyl-DMU 212 (DMU 291), (8) 3-desmethyl-DMU 212 (DMU 807) (9) DMU 212. The chromatogram is representative of three. For details of incubation, extraction and HPLC analysis, see Materials and Methods.