Figure 1
From: Quantitation of circulating DNA in the serum of breast cancer patients by real-time PCR
Real-time PCR. (A) A standard curve for the β-globin assay, using five-fold serial dilutions of genomic DNA (from 4000 to 1 genome equivalents). The X-axis denotes the threshold cycle (CT) and the Y-axis denotes the concentration of the target (logarithmic scale). (B) Amplification curves (triplicates) of three patients with breast cancer. (C) Amplification curves (triplicates) of three controls. The X-axis for (B) and (C) denotes the threshold cycle (CT) and the Y-axis denotes the Rn, which is the fluorescence intensity.
