Figure 3

Quantitative co-culture apoptosis assays. SW480 cells were pretreated with a FasL inhibiting recombinant protein rhFas:Fc prior to addition to mesothelial monolayers as co-cultures (CC). Monolayers (M) and SW480 tumour cells (SW) were cultured alone as controls. ± denotes pretreatment of cultures with recombinant protein. All co-culture experiments were terminated at 8 h. Mesothelial cell death is expressed as mean AI of triplicate experiments±s.d. ^*P<0.05, Mann–Whitney U-test.