Figure 2 | British Journal of Cancer

Figure 2

From: Osteopontin expression correlates with adhesive and metastatic potential in metastasis-inducing DNA-transfected rat mammary cell lines

Figure 2

An example of Southern hybridisation of C9-Met-DNA to DNA obtained from the clonal cell lines. (A) DNA from each of the cell lines was digested with EcoR1, electrophoresed on agarose gels, and blotted onto Hybond-N membrane. The samples loaded on the gel were 500 copies C9-Met-DNA (lane 1), 50 copies C9-Met-DNA (lane 2), Rama 37 DNA (lane 3), R37C9VM18 (lane 4), R37C9VM13 (lane 5), R37C9VM6 (lane 6), R37C9VM29 (lane 7), R37C9VM14 (lane 8), R37C9VM3 (lane 9), R37C9VM8 (lane 10), R37C9VM16 (lane 11) and R37C9VM25 (lane 12). The membrane was incubated with α32P-labelled C9-Met-DNA under hybridising conditions and subjected to autoradiography. The position of the authentic C9-Met-DNA is shown by the arrow. (B) Graph of C9-Met-DNA copy number (copy no) of the cell lines plotted against their metastatic potential in vivo (% Metastasis) (Materials and Methods). Results for the nine cloned C9-Met-DNA-transfected cell lines plus Rama 37 parental cells are shown.

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