Table 2 Relative levels of osteopontin and S100A4 mRNA and C9-Met-DNA copy number in the C9-Met-DNA transfectants

From: Osteopontin expression correlates with adhesive and metastatic potential in metastasis-inducing DNA-transfected rat mammary cell lines

Cell line

OPNamRNA

S100A4a mRNA

C9-Met-DNA copy numberb

R37

1c (±0.13)

1 (±0.23)

0

R37C9 VM3

11.1 (±0.55)c,d

1.6 (±0.15)

147 (±17)c,d

R37 C9 VM6

0.5 (±0.18)c

0.8 (±0.47)

0

R37 C9 VM8

1.4 (±0.14)c

1.2 (±0.19)

169 (±8)c,d

R37 C9 VM13

5.5 (±0.20)c,d

1.6 (±0.16)

133 (±10)c,d

R37 C9 VM14

8.0 (±0.5)c,d

0.6 (±0.38)

95 (±6)d

R37 C9 VM16

2.8 (±0.16)c,d

1.5 (±0.15)

8 (±3)d

R37 C9 VM18

4.5 (±0.24)d

0.8 (±0.05)

83 (±5)d

R37 C9 VM25

9.3 (±1.1)c,d

2.2 (±0.43)

145 (±17)c,d

R37 C9 VM29

0.7 (±0.21)c

1.3 (±0.34)

3 (±0.3)

R37 C9Pooled

4.3 (±0.28)d

0.8 (±0.14)

50d,e

  1. aRelative mRNA levels determined by Northern blotting.
  2. bC9-Met-DNA copy number determined by Southern blotting.
  3. cSignificantly different from C9-Met-DNA pooled transfectants (P<0.05; Student's t-test).
  4. dSignificantly different from Rama 37 (P<0.05; Student's t-test).
  5. eData from Chen et al (1997). Values in brackets represent the mean±standard deviation of three separate experiments.
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