Figure 2
From: Loss of NOS1 expression in high-grade renal cell carcinoma associated with a shift of NO signalling
Quantitative analysis of NOS1 mRNA in grade III clear cell RCC. Total RNAs were extracted from the tumour of four patients undergoing surgery for grade III clear cell RCC (T1–T4) and also from the corresponding normal renal parenchyma (N1–N4) taken at distance from the tumour, reversed transcribed and submitted to real time PCR as described in Material and Methods. Amounts of NOS1 amplification products were normalised to β-actin. In the four cases, NOS1 mRNAs were beyond the limit of detection in the tumours whereas they were always present in the corresponding normal renal parenchyma.
