Figure 6 | British Journal of Cancer

Figure 6

From: Isolation and characterisation of a human anti-idiotypic scFv used as a surrogate tumour antigen to elicit an anti-HER-2/neu humoral response in mice

Figure 6

Analysis of the Ab1′ response in sera of mice. Determination of anti-HER-2/neu IgG or IgM or total Ig levels in sera collected at various times after immunisation with (A) soluble purified anti-Id scFv, protocol 1; (B) phage-displayed scFv, protocol 2; (C) HER-2/neu ECD-Fc fusion protein. Mice were immunised with these antigens at the indicated times (arrows). Bound antibodies in sera diluted 1 : 100 were detected with HRP-conjugated anti-total Ig or μ-or γ-chain-specific anti-mouse Ig. Data are presented as mean±s.d. A490 values of five determinations, corresponding to the five mice in each group. Curves indicate sera from mice immunised with PBS (⋄), sera from mice immunised with anti-Id scFv 40 (□), sera from mice immunised with anti-Id scFv 69 (▵), and sera from mice immunised with HER-2/neu ECD-Fc (○).

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