Table 1 Primer sequences and PCR conditions for the ATP-binding cassette transporter (ABC) genes that were located in the amplified chromosomal regions, BCRP1, MRP1, MRP2, MRP5 and the control genes hypoxanthine phosphoribosyltransferase (HPRT) and β-2 microglobulin, used for DNA amplification in the RT–PCR
From: Mitoxantrone resistance in a small cell lung cancer cell line is associated with ABCA2 upregulation
Gene | Sense primer (5′ → 3′) | Antisense primer (3′ → 5′) | Temperature (°C) | Cycles (n) | Product size (base pairs) |
|---|---|---|---|---|---|
ABCB6 | GGG CCG TAT TGA GTT TGA GA | ATG TCC TGC CCA TCT ATT CG | 95, 55, 72a | 26 | 201 |
ABCC10 (MRP7) | CTC CCA CTG GAT CTC TCA GC | TCG CAT ACA CGG TGA GGT AG | 95, 57, 72a | 30 | 200 |
ABCF1 (ABC50) | GGA GTA CAC TGT GCG CTT CA | TCA GCA GCA GGA GTA GCG TA | 95, 55, 72a | 26 | 196 |
ABCA2 (ABC2) | GAG ATC CGC AGA GAG ATG GA | CTT CAG GAT GAG GTC CCA GA | 95, 57, 72a | 30 | 207 |
ABCC4 (MRP4) | AAT ACC CTT GGT TCC CCT TGG | ATC CTG GTG TGC ATC AAA CA | 95, 55, 72a | 32 | 202 |
ABCG2 (BCRP1) | CAG AGA TCA TAG AGC CTT CC | ACA CTC TGT AGT ATC CTC TG | 95, 55, 72a | 32 | 453 |
ABCC1(MRP1) | AAT GCG CCA AGA CTA GGA AG | ACC GGA GGA TGT TGA ACA AG | 95, 56, 72a | 29 | 990 |
ABCC2 (MRP2) | CTG GTT GAT GAA GGC TCT GT | CTG CCA TAA TGT CCA GGT TC | 95, 58, 72a | 31 | 1067 |
ABCC5 (MRP5) | GGA TAA CTT CTC AGT GGG | GGA ATG GCA ATG CTC TAA AG | 95, 55, 72a | 29 | 380 |
HPRT | CGT GGG GTC CTT TTC ACC AGC AAG | AAT TAT GGA CAG GAC TGA ACG TC | 95, 55, 72a | 27 | 380 |
β-2 microglobulin | CCA GCA GAG AAT GGA AAG TC | GAT GCT GCT TAC ATG TCT CG | 95, 55, 72a | 20 | 268 |
