Figure 2
From: Molecular alterations in apoptotic pathways after PKB/Akt-mediated chemoresistance in NCI H460 cells
Decreased chemosensitivity of NCI H460-Akt1 cells towards doxorubicin, cisplatin, and Mitoxantrone. Control transfected NCI H460 cells (filled squares) or NCI H460-Akt1 cells (open triangles) were seeded in 96-well plates in medium containing 0.5% serum. After 24 h cells were treated with different concentrations of doxorubicin, cisplatin, or Mitoxantrone as indicated and incubated for another 72 h at 37°C. Cell viability was then determined with a standard XTT assay as described in ‘Materials and methods’. Values are the mean of at least three independent experiments.
