Figure 3

(A) Activation of initiator caspases after treatment of NCI H460 cells with cisplatin. Control transfected NCI H460 cells or NCI H460-Akt1 cells were grown in medium containing 0.5% serum overnight and afterwards treated with cisplatin (20 μ M) for 36 h. Cell lysates were analysed by immunoblotting with antibodies specific for caspase 8 or caspase 9 as indicated. (B) Activation of effector caspases and PARP after treatment of NCI H460 cells with cisplatin. Lysates from cells treated as described above were prepared and analysed by immunoblotting with antibodies specific for caspase 3, caspase 7, and PARP as indicated. (C) Enzymatic assays of caspase activity. Cells were treated as described above. Additionally, samples from cells treated with 400 nM Mitoxantrone for 24 h were included. Caspase 3, 8, and 9 activities from lysates were determined by a colorimetric assay as described in ‘Materials and methods’.