Table 1 Binding of biotinylated CM79 to MFECP, MFEdmCP and MFECPHis

From: Modifying an immunogenic epitope on a therapeutic protein: a step towards an improved system for antibody-directed enzyme prodrug therapy (ADEPT)

Fusion protein

CM79 bindinga (%)

MFECP (no tag)

100

MFEdmCP (no tag)

0.8b

MFECPHis (His-tag)

54b

MFEdmCPHis (His-tag)

0.9b

  1. aMeasured by ELISA on CEA-coated plates. Binding of biotinylated CM79 was detected by Extravidin AP.
  2. bBinding of MFEdmCP, MFECPHis and MFEdmCPHis expressed as percentage of binding of MFECP.
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