Table 1 Primers and restriction enzymes for ATM exon and SNP analysis
From: ATM polymorphisms as risk factors for prostate cancer development
ATM exon or SNP | Technique | Primers | Restriction enzyme and digestion conditions |
|---|---|---|---|
Exon 39 | DHPLC | Forward: 5′-GGCAGATTAATCTATCATCTTTTAGA-3′ | |
Reverse: 5′-ATTCTGTTTCATTATGGTAATGGC-3′ | |||
Fragment size 372 bp | |||
Exon 24 | DHPLC | Forward: 5′-TTCATATTCAACCACAGTTC-3′ | |
Reverse : 5′-TGTAAGACATTCTACTGCCATC-3′ | |||
Fragment size 224 bp | |||
3161C>G (P1054R) | RFLP | Forward: 5′-AGCACAGAAAGACATATTGGAAG-3′ | o/n at 37°C 10 U AlwI |
Reverse: 5′-ACTATGTAAGACATTCTACTGCC-3′ | |||
Fragment size 494 bp |
