Figure 2

Pan-RAR antagonist AGN194310 affects the growth of prostate carcinoma cells more than that of normal prostate epithelium. The top panel shows the activity of the pan-RAR antagonist AGN194310 against LNCaP cells (closed circles), PC-3 cells (closed triangles), DU-145 (squares) a patient's primary carcinoma cells (open triangles) and normal prostate epithelium (open circles). The lower panels show the activities of antagonists of RARβγ (AGN194431) and RARα (AGN196996) against LNCaP cells (closed circles) and normal prostate epithelium (open circles). Activities were measured by seeding cells into wells of a microtitre plate, treating with agents immediately and at day 2, and measuring cellular ATP levels at day 5. Data are means±s.e. of values obtained from six (A), four (B) and three (C) experiments. The P-values obtained when the IC₅₀ value for AGN194310 against normal prostate epithelium was compared with IC₅₀ values against LNCaP, PC-3 and DU-145 cells are 0.008, 0.001 and 0.009, respectively. Comparison of the IC₅₀ values for AGN194431 against normal prostate epithelium and LNCaP cells gave a P-value of 0.1, and a P-value of 0.4 was obtained for this comparison for AGN196996.