Figure 5

LNCaP cells undergoing AGN194310-induced apoptosis bind Annexin V. LNCaP cells were treated with 1 μ M AGN194310, or vehicle control (DMSO) and stained with Annexin V and propidium iodide. Fluorescence microscopy at day 3 (A) reveals early apoptotic cells (Annexin V +ve; green), as well as late apoptotic/necrotic cells (Annexin V +ve/propidium iodide +ve; yellow-red) in AGN194310-treated cultures. (B) Representative FACS cytograms of stained cells. Viable cells (Annexin V and propidium iodide −ve) are in the lower left-hand quadrant. Early apoptotic cells (Annexin V +ve/propidium iodide −ve) are in the lower right-hand quadrant. Terminal apoptotic/necrotic cells (Annexin V +ve/propidium iodide +ve) are in the upper right-hand quadrant. (C) Results of the flow cytometry analyses are summarised. Data are means±s.e. of values from at least three experiments. ^**Denotes P-values <0.001.