Figure 4

Overexpression of Apaf-1 sensitises melanoma cells to anticancer drug treatment. (A) Western blot showing endogenous level of Apaf-1 parental MMRU and MMRU cells transfected with Apaf-1. (B) Decreased cell survival of melanoma cells overexpressing Apaf-1. MMRU cells were either transfected with control GFP or Apaf-1 cDNAs followed by various drug treatment: DOX (500 nM), VIN (800 nM), or CPT (200 nM) for 24 h, and the cell survival rate was determined by SRB assay. (C) Increased apoptosis of melanoma cells overexpressing Apaf-1. MMRU and MEWO cells were transfected with GFP or Apaf-1, and then treated with 200 nM CPT for 24 h followed by ELISA assay of apoptosis.