Figure 1 | British Journal of Cancer

Figure 1

From: Sustained antiproliferative mechanisms by RB24, a targeted precursor of multiple inhibitors of epidermal growth factor receptor and a DNA alkylating agent in the A431 epidermal carcinoma of the vulva cell line

Figure 1

Selective inhibition of EGFR autophosphorylation in intact cells by RB24. Serum-starved A431 cells were preincubated for 2 h with the indicated concentrations of RB24 prior to stimulation with EGF for 15 min. An equal amount of cell lysates was analysed by Western blotting using antiphosphotyrosine antibodies. Membranes were stripped of antiphosphotyrosine and reprobed with anti-EGFR antibodies as a loading control. Band intensities were measured using the SynGene GeneTools software package.

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