Figure 4

Modulation of VEGFR2 by VEGF in vivo. The VEGF121 (15 μg/mouse) was administered via tail vein in mice, and their lungs harvested at the indicated time points. Values represent the ratio of VEGFR2 to β-tubulin as determined by densitometry and normalised to vehicle-treated mice. Human umbilical vein endothelial cells immunoprecipitated using anti-VEGFR2 antibody from 350 μg protein were used as controls.