Figure 4

Treatment of Mia PaCa2 cells with 1 μg ml⁻¹ cytochalasin D. Culture of Mia PaCa2 cells for 72 h in the presence of cytochalasin D resulted in a 24-fold increase in IL-8 secretion on fibronectin-coated wells and a 31-fold increase in IL-8 secretion on BSA-coated control wells (A). Cytochalasin treatment also inhibited proliferation in fibronectin-coated wells in comparison with no treatment or treatment with the cytochalasin carrier vehicle DMSO (B). When IL-8 levels were expressed as a fraction of the percentage change in cell numbers, a large increase in secretion was still apparent and remained lower on fibronectin than on BSA. Fibronectin-induced cell spreading was prevented by cytochalasin (D). ^*P<0.05 vs untreated control.