Figure 2
Western blot analysis of transgenic c-Myc protein expression and Bax protein expression in mammary tumours and mammary gland tissues from parous MMTV-c-myc transgenic/bax-knockout mice. Protein lysates were prepared from mammary tumours (T lanes), mammary gland tissues (MG lanes), and from the A-20 murine lymphoblast cell line (Bax protein positive control). (A) Western blot analysis demonstrates expression of c-Myc protein only in mammary tumour lysates from c-myc transgene-positive parous study mice. (B) Western blot analysis demonstrates that expression of Bax protein is lost in tumour and mammary gland tissue lysates from bax-nullizygous parous study mice, that expression of Bax protein is reduced in mammary gland tissue lysates from bax-hemizygous parous study mice as compared with bax-intact mice, and confirms that bax loss of heterozygosity is not evident in mammary tumours from parous MMTV-c-myc transgenic/bax-hemizygous study mice (Western blots were run simultaneously under identical conditions with mammary gland tissue lysates equally loaded for the amount of protein).
