Figure 4

Release of cytochrome c to the cytosol in PC3 cells treated with 75 μ M DIM for 8–72 h. Cells were treated with DIM and their cytosol protein fraction was extracted, separated on SDS–PAGE and exposed to specific antibody using Western blotting, as described in ‘Materials and methods’. (A) Western blotting results. C* denotes a representative control group. The figure shown is a representative of three independent experiments. (B) Densitometer results. Data presented are averages of three independent experiments (±s.e.) and are expressed as percentages of the respective controls. *P<0.05; ***P<0.001.