Figure 1
From: Sp17 gene expression in myeloma cells is regulated by promoter methylation
Reverse transcription–polymerase chain reaction analysis using a pair of sequence-specific primers for Sp17 produced a positive signal of approximately 500 bp (lane 1=ARK-B; lane 2=ARP-1; lane 3=H929; lane 4=IM-9; lane 5=KMS-11; lane 6=MM1-R; lane 7=U266; and lane 8=RPMI 8226) (M=molecular marker; lane a=PCR without prior RT; lane b=RT–PCR for Sp17; lane c=control amplification for a β-actin gene segment).
