Figure 2
From: Sp17 gene expression in myeloma cells is regulated by promoter methylation
Polymerase chain reaction analysis for Sp17 promoter sequence using six primer pairs to amplify across eight HpaII sites. Therefore, PCR products were only obtained if the target sequence are methylated at the HpaII sites (A=PCR without prior HpaII digest; B=PCR after HpaII digest) (lane 1=ARK-B; lane 2=ARP-1; lane 3=H929; lane 4=IM-9; lane 5=KMS-11; lane 6=MM1-R; lane 7=U266; and lane 8=RPMI 8226) (M=molecular marker).
