Figure 4
From: Sp17 gene expression in myeloma cells is regulated by promoter methylation
Analysis of 293 cells for CAT activities after plasmid transfection to confirm the role of Sp17 promoter methylation (lane 1=wild-type 293 cells; 293 cells transfected with pCAT*3 Enhancer-Sp17 promoter; lane 3=293 cells transfected with the positive control plasmid pCAT*3 Enhancer-SV40 promoter; lane 4=293 cells transfected with a control plasmid, pcDNA3.1; lane 5=293 cells transfected with pCAT*3 Enhancer plasmid that did not contain any promoter; lane 6=293 cells transfected with pCAT*3 Enhancer-Sp17 promoter/met; lane 7=product control; lane 8=substrate control).
