Table 1 Oligonucleotide primers used for methylation-sensitive PCR analysis
From: Sp17 gene expression in myeloma cells is regulated by promoter methylation
Primer | Primer sequences | Gene segment amplified HpaII sites | Annealing temperature (°C) |
|---|---|---|---|
I | 5′-CAC CCT TTT GCA GTC CTC GCA GC-3′ | From position −186 to −28 | 60 |
5′-CTC CTT TCT CTC CGA GCT GGT GC-3′ | (IA and IB) | ||
II | 5′-CGC GAC CAT GCT TGC AAG GA-3′ | From position –280 to –62 | 52 |
5′-TTG TTT TTC TAG TTG CTG GG-3′ | (II) | ||
III | 5′-GCC GCC TCA CCT GCA CTT CTG TC-3′ | From position –348 to –227 | 60 |
5′-GAA CCC ACT TTC CCA GTC CTA GG-3′ | (III) | ||
IV | 5′-GCC TCG GTC GGA GAC GCG AGC CC-3′ | From position –488 to –285 | 60 |
5′-GGC TTG TAC TCG GGC TGG TGC TG-3′ | (IVA and IVB) | ||
V | 5′-GGT TCA ACC CAG CAG ACT CCT GC-3′ | From position –518 to –362 | 60 |
5′-ACC CCT GCC CCT CAG CCT GTG CG-3′ | (V) | ||
VI | 5′-ACC TAA CTA GCT TCG CCT CCT TC-3′ | From position –693 to –493 | 60 |
5′-TCT GCA GGA GTC TGC TGG GTT GA-3′ | (VI) | ||
Control | 5′-GGG ACA CAT AGC CAg AGA G-3′ | Exon 5, position 377 to 559 | 52 |
5′-TTC ACA ACA GTA ACG AAT G-3′ |
