Figure 1
Effect of SN50 (18 μ M) (lanes 6–10) and the cell permeable inactive control peptide SN50M (18 μ M) (lanes 1–5) on PIF-induced expression of 20S proteasome α-subunits (A), MSS1 (B), p42 (C), E214k (D) and myosin (E) in murine myotubes 24 h after addition of PIF, as determined by Western blotting. The peptides were added 2 h prior to PIF. An actin loading control is shown in (F). Myotubes were treated with 0 (lanes 1 and 6), 2.1 (lanes 2 and 7), 4.2 (lanes 3 and 8), 10.5 (lanes 4 and 9) and 16.8 nM PIF (lanes 5 and 10). A densitometric analysis presenting the average of three separate blots is shown for each Western blot. The densitometric analysis of the proteasome 20S α-subunits is for the two major bands in the absence (▪, 
) and presence (□, 
) of SN50 (18 μ M). Values for the control peptide are shown as solid boxes and for SN50 as open boxes. Differences from control are indicated as a, P<0.05, b, P<0.01 and c, P<0.001, while differences from the control peptide in the presence of SN50 are shown as d, P<0.05, e, P<0.01 and f, P<0.001.
