Figure 3
Effect of curcumin on PIF-induced expression of 20S proteasome α-subunits (A) and E214k (B) in murine myotubes, determined by Western blotting. Cells were incubated for 24 h with 0 (lanes 1 and 6), 2.1 (lanes 2 and 7), 4.2 (lanes 3 and 8), 10.5 (lanes 4 and 9) or 16.8 nM PIF (lanes 5 and 10) in the absence (lanes 1–5) or presence (lanes 6–10) of curcumin (50 μ M) added 2 h prior to PIF. An actin loading control is shown in (C). Densitometric analysis of three separate blots is shown under each Western blot. Differences from 0 nM PIF are shown as a, P<0.05, b, P<0.01 and c, P<0.001, while differences in the presence of curcumin are indicated as d, P<0.05, e, P<0.01 and f, P<0.001.
