Figure 5
(A) Effect of resveratrol (30 μ M) on total protein degradation in murine myotubes in the presence of PIF for 24 h, as measured by the release of [3H]phenylalanine. Myotubes were incubated with PIF alone (♦) or preincubated with resveratrol 2 h prior to addition of PIF and maintained in the culture medium over the 24 h period (×). Differences from control are indicated as b, P<0.01 and c, P<0.001, while differences in the presence of resveratrol are indicated as f, P<0.001. The experiment was repeated three times (n=9). (B) Chymotrypsin-like enzyme activity in soluble extracts of murine myotubes after treatment with PIF alone for 24 h (solid boxes) or after treatment with PIF in the presence of resveratrol (30 μ M) (open boxes). The symbols for the differences are the same as in (A). The experiment was repeated three times (n=9).
