Figure 7
Effect of PIF on I-κBα expression (A) and nuclear translocation of NF-κB, as determined by EMSA (B) in murine myotubes 30 min after the addition of PIF. An actin loading control for (A) is shown in (C). Myotubes were incubated with 0 (lanes 1 and 6), 2.1 (lanes 2 and 7), 4.2 (lanes 3 and 8), 10.5 (lanes 4 and 9) or 16.8 nM PIF (lanes 5 and 10) in the absence (lanes 1–5) or presence (lanes 6–10) of resveratrol (30 μ M). In (B), lane 12 is a positive control for NF-κB (supplied by the manufacturer of the kit), while lane 11 contains the positive control for NF-κB together with a 100-fold excess of unlabelled NF-κB probe. The densitometric analysis represents three separate experiments. Differences from control are indicated as b, P<0.01 and c, P<0.001, while differences in the presence of resveratrol are indicated as e, P<0.01 and f, P<0.001.
