Figure 5
Effect of DMF diet on MMC toxicity to platelets in CD-1 nude mice. CD-1 nude mice were implanted with HCT116 cells subcutaneously in the right flank of each mouse. After 7–10 days the mice were fed a diet containing 0 or 0.3% DMF for 7–10 days. The mice were weighed and received a single tail-vein injection of saline (control diet only), 2.0 mg kg−1 MMC (control diet and 0.3% DMF diet) or 3.5 mg kg−1 MMC (control diet only). On various days approximately 20 μl of blood was collected from the saphenous vein of some of the mice in each treatment group. The platelet counts were determined with a Coulter Z2 particle counter and cell analyser. The points represent the mean platelet counts±s.e. of 2–14 mice. Differences in the platelet counts in different treatment groups were analysed statistically by ANOVA for each time point.
