Figure 1
Colonies of hygromycin-resistant prostate epithelial cells expressing clusterin are smaller than those expressing only hygr. Cell colonies selected in cell culture dishes, fixed with paraformaldehyde 4% and stained with Giemsa. Note the smaller size of the clu colonies (clu) compared to the hyg colonies (hyg), after 14 days of selection with hygromycin B. The figure shows a representative result obtained by performing the experiment four times using different standard endo-free plasmid preparations.
