Figure 1
From: Inhibition of AKT survival pathway by a small molecule inhibitor in human endometrial cancer cells
Expression of PTEN and phospho-AKT in endometrial cancer cell lines. Cell lysates were immunoblotted with phosphorylated AKT (Ser 473 or Thr 308), phosphorylated SGK (Ser 78), total AKT, or PTEN antibody. The same lysates were immunoprecipitated with anti-AKT antibody; AKT kinase assay was performed with GSK-3 as a substrate and phospho-specific GSK-3 α/β (Ser 21/9) for phosphorylated protein detection.
