Figure 4

(A) Relative expression of SLIT3 gene in methylated (M) and unmethylated (U) breast tumours using real-time RT–PCR as described in Materials and Methods (B) BstUI digest of SLIT3 CpG island COBRA PCR products from glioma (T208, T116, T45) and colorectal (T46, T49) primary tumours. (C) COBRA PCR products from glioma and colorectal primary tumours were cloned and sequenced. For each tumour, several clones were sequenced and the methylation status for the first 30 CpGs is shown. White and black squares represent unmethylated and methylated CpGs, resepectively. Partially filled squares represent partially methylated CpGs. 7T and 3T represent colorectal tumours, 7N and 3N represent corresponding normal colon samples. 209T and 205T represent glioma tumours.