Figure 1

(A) Cell count before and after 72 h dose-escalating CP-320626 treatment of MIA pancreatic adenocarcinoma cells in culture. Cell numbers before the study and 72 h later in response to 50 μ M CP-320626 treatment are not statistically different from the starting cell number. Control cells were grown for 72 h with vehicle only. (B) TUNEL assay indicates 4% apoptotic MIA cells formation in 50 μ M CP-320626-treated cultures, which increases about seven-fold, to 28%, after 100 μ M CP-320626 treatment. (C) Cell count before and after 72 h dose-escalating CP-320626 treatment of CRL-1501 human fibroblasts. (D) TUNEL assay indicates only 1–5% apoptotic CRL-1501 cell formation in 50 μ M CP-320626-treated cultures. (n=6; mean+s.d.; ^*P<0.05, ^**P<0.01).