Figure 3

SIDMAP of MIA cells after treatment with increasing doses of the glycogen phosphorylase inhibitor CP-320626 indicates severe changes in intracellular glucose carbon distribution. Carbon dioxide release from glucose is decreased, as demonstrated by a fall in ¹³C/¹²C ratios in panel A; the decrease in pentose cycle direct glucose oxidation and recycling into lactate is shown in panel B as per cent (%) of glycolysis; the decrease in nucleic acid (RNA) ribose ¹³C molar enrichment is shown in panel C as ¹³C ∑m_n, and the severe decrease in TCA cycle anabolic glucose use (anaplerosis) is shown in panel D as per cent (%) of glucose oxidation within the cycle (n=6; mean+s.d.; ^*P<0.05, ^**P<0.01).